Coding
CXCR4-T2A

Part:BBa_K1993032

Designed by: Su Xiaojun   Group: iGEM16_SYSU-MEDICINE   (2016-10-13)


CXCR4-T2A-Luciferase

With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing coding sequences of chemokine receptor CXCR4 and bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology.

CXCR4 is responsible for directing T lymphocytes to the lesions whose ligand is CXCL12, one of the most common chemokines in various inflammatory diseases, especially in inflammatory bowel disease(IBD). (Details could be seen on BBa_K1993013).

Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us to observe cells in vivo. (Details could be seen on BBa_K1993015).

T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on BBa_K1993019)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1350
    Illegal BamHI site found at 494
    Illegal BamHI site found at 1893
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None